ELISA kits come highly recommended in scientific, research and clinical tests because of their detection ranges and their sensitivities. The two main kinds of ELISA assays carried out using the ELISA kits are the sandwich and the competitive ELISA assay kits that have high precision levels.
These enzyme-linked immunosorbent assay testing methods are effective in diagnosing diseases, medical research, detecting of banned substances by anti-doping agencies, and commercial food companies in production of allergen-free food items.
The main types of ELISA kits available in the market include:
1. The Rituximab ELISA kit
Rituximab is a chimeric monoclonal antibody that is used in diseases characterized by excess B cells. It targetsCD20, a protein expressed on the surface of B cells inducing apoptosis in B cells. It is therefore used in treatment of autoimmune disorders, Leukemia, and lymphomas.
The Rituximab ELISA kit is highly specific and sensitive and therefore, it has been designed to measure the Rituximab levels. The sandwich ELISA assay is used for this antibody.
2. Zika Virus IgM ELISA kit
This is an effective mybiosource ELISA testing kit designed to qualitatively analyze the existence or the absence of the human Zika virus IgM in human plasma, serum, and any other biological fluids. The spread of the virus is through Aedes mosquito bites. The mosquitoes bite during the day and at night. It employs the sandwich ELISA testing procedure to detect the virus antibodies.
3. Diphtheria IgG ELISA kit
This assay kit has been designed to detect and to qualitatively determine the presence of the diphtheria toxoid in plasma and blood serum. Diphtheria is caused by the Corybacteriumdiphtheriae and the antibodies producedfollowing its introduction in the body are tested using the kit. The Toxoid IgG is the antibody detected in the assay.
4. Human Zonulin ELISA kit
This is used in research institutions to determine the presence of the native zonulin in biological samples. The test is based on antibody-zonulin antigen interactions, i.e. immunosorbency, and the HRP colorimetric detection systems. Zonulin is believed to be the cause of the leaky gut because it opens up spaces between the cells of the intestinal wall lining. This normally allows normal passage of nutrients and other biomolecules. However, in the leaky gut, the spaces open up to the extremes leading to immunological reactions.
5. LTA/ Lipoteicholic Acid ELISA kit
The test is based on the competitive ELISA test that quantitatively determines the target antigen levels in homogenates, tissue fluids, plasma, serum and cell culture supernates.
Lipoteichoic Acid is a surface-associated adhesion amphiphile obtained from Gram-positive bacteria. It serves as the regulator of the autolytic wall enzymes, the muramidases. Bacteriolysis induced by lysozymes or cationic peptides in leucocytes cause release LTA. It is known to induce arthritis, encephalomyelitis, uveitis, nephritis, periodontal lesions, and meningeal inflammations. ELISA tests determine the levels of the antigen in body fluids.
6. PINP ELISA kit
PINP, or the Procollagen I N-Terminal Propeptide is derived from collagen type I, the most common type of collagen in mineralized bone, collagen is synthesized by osteoblasts as procollagen made of the PINP and the carboxyl-terminal propeptide. PINP is a sensitive marker for bone formation and is used in monitoring of bone formation therapies. The PINP ELISA test kits help to detect the levels of PINP in serum, biological fluids, and plasma.
In conclusion, these ae some of the main ELISA test kits available for research rather than diagnostic purposes. The accuracy and precision of the results is high and the results can be reproduced.